中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (29): 5433-5436.doi: 10.3969/j.issn. 2095-4344.2012.29. 023

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

可塑型脱矿骨基质/海藻酸钠复合材料的制备及细胞相容性

徐伟俊，李宝兴，张育敏，刘晓明，李 靖，王旭昇，马绍英   

  1. 中国辐射防护研究院，山西省太原市 030006
  • 收稿日期:2011-12-28 修回日期:2012-01-28 出版日期:2012-07-15 发布日期:2012-07-15
  • 作者简介:徐伟俊★，男，1985年生，浙江省武义县人，汉族，2011年中国辐射防护研究院毕业，硕士，主要从事生物组织移植材料的研发。 zymsir@sina.com

Preparation and cell compatibility of demineralized bone matrix/sodium alginate composite

Xu Wei-jun, Li Bao-xing, Zhang Yu-min, Liu Xiao-ming, Li Jing, Wang Xu-sheng, Ma Shao-ying   

  1. China Institute for Radiation Protection, Taiyuan 030006, Shanxi Province, China
  • Received:2011-12-28 Revised:2012-01-28 Online:2012-07-15 Published:2012-07-15
  • About author:Xu Wei-jun★, Master, China Institute for Radiation Protection, Taiyuan 030006, Shanxi Province, China zymsir@sina.comg

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447

被引次数

12

摘要：

背景：制备具有良好骨传导、骨诱导活性、细胞相容性，且可任意塑形的骨修复材料，对于不规则骨缺损以及难愈合性骨折的治疗具有重要意义。
目的：构建可塑型脱矿骨基质/海藻酸钠复合组织工程骨材料，筛选出最佳复合比例并评价其细胞相容性。
方法：选取健康供体皮质骨，经清洗、粉碎、脱钙处理制备脱矿骨基质，以5/5，6/4，7/3，8/2比例与海藻酸钠复合，通过测定离散直径选取合适的比例构建脱矿骨基质/海藻酸钠复合材料。将所制备脱矿骨基质/海藻酸钠材料浸提液与L-929小鼠成纤维细胞复合培养，2，4，7 d后经MTT法检测细胞毒性。
结果与结论：随着海藻酸钠含量的增加，复合材料黏性变强，水中离散直径逐步减小，当脱矿骨基质/海藻酸钠质量比为7/3时，复合材料可塑性强，不易离散，在37 ℃水浴中浸泡2 h的离散直径为3.7 cm，为复合的最佳比例，经MTT法检测，2，4，7 d的细胞增殖率分别为91.56%，95.43%，97.47%，细胞毒性级别均为1级。

关键词: 脱钙骨基质, 海藻酸钠, 离散度, 细胞相容性, 骨修复材料, 生物材料

Abstract:

BACKGROUND: It is important to prepare a bone substitute material with good osteoacusis, osteoinductive activity and cell compatibility that is easily molded, which is of significance to repair irregular bone defects and refractory bone fractures.
OBJECTIVE: To prepare demineralized bone matrix/sodium alginate (DBM/ALG) composite material and to screen out the optimal composite ratio, as well as to evaluate its cell compatibility.
METHODS: DBM was prepared from the cortical bone of healthy donors by cleaning, grinding and demineralization. According to the ratio of 5/5, 6/4, 7/3, 8/2, the DBM was compounded with ALG, and the DBM/ALG composite material was prepared through screening the optimal ratio by disperse diameter method. L-929 fibroblasts of mice were cultured in the leaching liquor of DBM/ALG, and the cytotoxicity was tested by MTT method at days 2, 4 and 7.
RESULTS AND CONCLUSION: With the increase of ALG content, the viscosity of DBM/ALG was strengthened and dispersed diameter was reduced gradually. When the ratio of DBM/ALG was 7/3, DBM/ALG was not discrete easily, and dispersed diameter was 3.7 cm in 37 ℃ water. The results of MTT showed that cell proliferation rates were 91.56%, 95.43% and 97.47% at days 2, 4 and 7, respectively; while cytotoxicity was in grade 1.

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